What is Thin Layer Chromatography (TLC)?
Thin-layer chromatography (TLC) separates and analyzes chemical compounds on a thin layer of adsorbent material. The sample, applied near the base, moves up the plate with a solvent, revealing separated components. The separation occurs as a mobile phase, carrying the sample, moves through the stationary phase. TLC is known for its simplicity, speed, and cost-effectiveness in the qualitative analysis of mixtures. TLC is widely used in chemical analysis.
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Definition of TLC
Thin Layer Chromatography (TLC) is a chromatographic technique in which chemical compounds are separated and analyzed based on their differential migration over a thin layer of adsorbent material.
Thin Layer Chromatography
Thin layer chromatography in chemistry is a versatile and widely used chromatographic technique that plays a vital role in the field of analytical chemistry. Thin-layer chromatography (TLC) relies on the principle of differential migration of components in a mixture. TLC finds extensive application in chemistry for quick qualitative analysis, purity assessment, and compound separation.
In this article, we are going to discuss in detail Thin layer chromatography, its definition, the procedure to perform TLC, the principle of TLC, its advantages, disadvantages, and applications along with FAQs.
Table of Content
- What is Thin Layer Chromatography (TLC)?
- Principle of TLC
- TLC Diagram
- Phases in Thin Layer
- Thin Layer Chromatography Rf value
- Applications of TLC
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