TLC Diagram
Thin Layer Chromatography (TLC) works by spreading a thin layer of special material (like silica gel) on a plate.
- Take a glass filled with water. At the bottom of this glass is the solvent (water). Now, picture a paper strip with a black dot on it, which is our sample.
- When we put this strip in the glass, the solvent rises up.
- As the solvent rises up it carries the ink components with it.
- The different colored dye spots are separated on the TLC plate based on their affinity for the adsorbent material.
- This helps us see and understand what’s in the original sample.
Thin Layer Chromatography
Thin layer chromatography in chemistry is a versatile and widely used chromatographic technique that plays a vital role in the field of analytical chemistry. Thin-layer chromatography (TLC) relies on the principle of differential migration of components in a mixture. TLC finds extensive application in chemistry for quick qualitative analysis, purity assessment, and compound separation.
In this article, we are going to discuss in detail Thin layer chromatography, its definition, the procedure to perform TLC, the principle of TLC, its advantages, disadvantages, and applications along with FAQs.
Table of Content
- What is Thin Layer Chromatography (TLC)?
- Principle of TLC
- TLC Diagram
- Phases in Thin Layer
- Thin Layer Chromatography Rf value
- Applications of TLC
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