Procedure to perform TLC
The procedure for Thin Layer Chromatography (TLC) involves a few simple steps:
Step 1: Marking the TLC Plate: Use a pencil to make small marks at the base of the TLC plate where sample spots will be applied.
Step 2: Applying Sample Solutions: Apply the sample solutions to the marked areas on the TLC plate.
Step 3: Preparing the TLC Chamber: When adding the mobile phase to the TLC chamber, place a moistened filter paper in it to ensure a consistent humidity level.
Step 4: Placing the TLC Plate: After placing the TLC plate inside the chamber, cover it with the lid, ensuring the sample faces the mobile phase at all times.
Step 5: Immersing the Plate: Immerse the plate for development, keeping the sampling sites well above the mobile phase to avoid complete submersion.
Step 6: Waiting for Spot Appearance: Wait until spots start to appear on the TLC plate.
Step 7: Removing and Drying: Once stains become visible, carefully remove the plates and let them dry.
Step 8: Observation using UV Light: Utilize a UV light chamber to observe the sample spots.
Thin Layer Chromatography
Thin layer chromatography in chemistry is a versatile and widely used chromatographic technique that plays a vital role in the field of analytical chemistry. Thin-layer chromatography (TLC) relies on the principle of differential migration of components in a mixture. TLC finds extensive application in chemistry for quick qualitative analysis, purity assessment, and compound separation.
In this article, we are going to discuss in detail Thin layer chromatography, its definition, the procedure to perform TLC, the principle of TLC, its advantages, disadvantages, and applications along with FAQs.
Table of Content
- What is Thin Layer Chromatography (TLC)?
- Principle of TLC
- TLC Diagram
- Phases in Thin Layer
- Thin Layer Chromatography Rf value
- Applications of TLC
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