Principle of TLC
Thin-layer chromatography (TLC) is a chromatographic technique used for separating and analyzing mixtures. The principle involves
- Adsorption and migration of a sample mixture along a thin layer of adsorbent material.
- The separation occurs based on the differential affinity of the components in the mixture for the adsorbent and the mobile phase (solvent).
- As the mobile phase moves through the stationary phase, the components of the mixture move at different rates, resulting in distinct spots on the TLC plate.
- The Rf value (retention factor) is a key parameter used to characterize the separation of compounds in TLC.
Thin Layer Chromatography
Thin layer chromatography in chemistry is a versatile and widely used chromatographic technique that plays a vital role in the field of analytical chemistry. Thin-layer chromatography (TLC) relies on the principle of differential migration of components in a mixture. TLC finds extensive application in chemistry for quick qualitative analysis, purity assessment, and compound separation.
In this article, we are going to discuss in detail Thin layer chromatography, its definition, the procedure to perform TLC, the principle of TLC, its advantages, disadvantages, and applications along with FAQs.
Table of Content
- What is Thin Layer Chromatography (TLC)?
- Principle of TLC
- TLC Diagram
- Phases in Thin Layer
- Thin Layer Chromatography Rf value
- Applications of TLC
Contact Us