Difference between Paper and Thin Layer Chromatography
The difference between Paper and Thin Layer Chromatography is tabulated below:
|
Paper Chromatography Vs Thin Layer Chromatography |
|
|---|---|
|
Paper Chromatography |
Thin Layer Chromatography |
|
It works on principle of partition. |
It works on principle of adsorption. |
|
It consumes more time. |
It consumes less time. |
|
Paper chromatography is more efficient in case of polar and water soluble compounds. |
TLC performs better separation in case of less polar compounds. |
|
It cannot be examined under UV. |
It can be examined under UV. |
|
Less physical strength. |
More physical strength. |
|
Paper can’t be heated for a long time. |
TLC plates can be heated for a long time. |
|
Silica and alumina cannot be used. |
Plates used for TLC are prepared by coating thin layers of alumina or silica on glass plates. |
|
Corrosive reagents destroy the paper. |
Plates may be coated with corrosive reagents. |
Thin Layer Chromatography
Thin layer chromatography in chemistry is a versatile and widely used chromatographic technique that plays a vital role in the field of analytical chemistry. Thin-layer chromatography (TLC) relies on the principle of differential migration of components in a mixture. TLC finds extensive application in chemistry for quick qualitative analysis, purity assessment, and compound separation.
In this article, we are going to discuss in detail Thin layer chromatography, its definition, the procedure to perform TLC, the principle of TLC, its advantages, disadvantages, and applications along with FAQs.
Table of Content
- What is Thin Layer Chromatography (TLC)?
- Principle of TLC
- TLC Diagram
- Phases in Thin Layer
- Thin Layer Chromatography Rf value
- Applications of TLC
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