Separation and isolation of DNA fragments
The cutting of DNA by restriction endonucleases results in fragments of DNA. These fragments can be separated by a technique referred to as gel electrophoresis. the component of each reaction take separated to determine the length of each strand. It consists of a thin layer of gel made of polyacrylamide and 5 ml of DNA placed onto the gel strand. Because DNA fragments are negatively charged molecules, they can be separated by forcing them to move toward the anode during a medium/matrix. Agarose is one of the components of Agar that is a mixture of polyacrylamide isolated from certain seaweeds. The DNA fragments separate (resolve) as per their size during the sieving effect provided by the agarose gel. The separated DNA fragments can be exposed only after staining the DNA with a compound referred to as ethidium bromide dye followed by highlighting to UV radiation. In a UV-exposed ethidium bromide-stained gel, bright orange bands of DNA can be seen. The separated DNA bands are sliced off of the agarose gel and removed from the gel component. This process is called elution. The purified DNA fragments are utilized to create recombinant DNA by combining them with cloning vectors.
NCERT Notes Biology Class 12 Chapter 9 Biotechnology: Principles and Processes
NCERT CBSE Class 12th Science Notes Chapter 9 Biotechnology: Principles and Processes: Biotechnology Principles and Processes is an important part of Class 12 Science Notes for quick revision. They will benefit from having challenging study material to use in preparing for the exam. Students can get CBSE Class 12th Science Notes Chapter 9 Biotechnology: Principles and Processes, Biotechnology, the principle of biotechnology, Recombinant DNA Technology, Separation and Isolation of DNA Fragments, Tools of Recombinant DNA Technology.
The branch of biology known as biotechnology is where technology is used to improve human health. Biotechnology is the production, development, and modification of useful products to satisfy the needs of all living things.
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