Obtaining the Foreign Gene Product
In recombinant technologies, the desired gene is selected, followed by a selection of an ideal vector in which the specific gene is integrated, and then the gene of interest is fused with the vector to form recombinant DNA. Once this foreign DNA is integrated, the host multiplies and eventually produces the desired protein. The rDNA must be retained in the host and passed on to the progeny. In order for the desired protein to be produced, the gene that encodes for it needs to be expressed. It occurs only under optimized conditions. Not only the target protein has to be expressed but it has to be produced on a large scale.
Recombinant cells can be multiplied extensively using a continuous culture system. Here the cells are cultured in a sizable vessel and the medium is refreshed at regular changes to maintain optimum conditions. This aid to culture a large quantity of the desired protein. This can be achieved by utilizing a bioreactor.
NCERT Notes Biology Class 12 Chapter 9 Biotechnology: Principles and Processes
NCERT CBSE Class 12th Science Notes Chapter 9 Biotechnology: Principles and Processes: Biotechnology Principles and Processes is an important part of Class 12 Science Notes for quick revision. They will benefit from having challenging study material to use in preparing for the exam. Students can get CBSE Class 12th Science Notes Chapter 9 Biotechnology: Principles and Processes, Biotechnology, the principle of biotechnology, Recombinant DNA Technology, Separation and Isolation of DNA Fragments, Tools of Recombinant DNA Technology.
The branch of biology known as biotechnology is where technology is used to improve human health. Biotechnology is the production, development, and modification of useful products to satisfy the needs of all living things.
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