High-Performance Liquid Chromatography
High-Performance Liquid Chromatography also called HPLC Chromatography is a method in analytical chemistry to separate the components of a mixture and to identify and measure their quantity. This method was introduced by M.S. Tswett who was a botanist. A sample has two types of components named analyte and matrix. It is the analyte that we want to identify and quantify and the rest of the other components of the sample are called the Matrix.
In chromatography, the sample is introduced in the mobile phase which is a liquid that passes through a solid which is usually a solid or a liquid on the solid. In HPLC, the stationary phase is in a column packed with small porous particles and the liquid passes through it with the help of a pump. The sample then passes through the column. The particles of the sample move at different rates due to the varying nature of interaction with the stationary phase. After the sample crossed the column, the substances are identified and quantified using the computer and the software attached to it.
The different types of HPLC include Normal Phase HPLC, Reverse Phase HPLC, Ion Exchange HPLC, and Size Exclusion HPLC.
HPLC is used in the analysis of drugs, synthetic polymers, environmental particles, water purification, and isolation of various valuable products.
Chromatography
Chromatography is the separation of mixtures by distribution between two or more immiscible phases. In chemical laboratories, where it is used for analysis, isolation, and purification, chromatography is the most used separation technique. It is also frequently used in the chemical process industry as a part of small and large-scale production.
There are many types of Chromatography, e.g. Adsorption Chromatography, Thin Layer Chromatography, Column Chromatography, Partition Chromatography, etc. Paper Chromatography is an example of Partition Chromatography. Let’s learn more about Chromatography, its meaning, principle, types, and applications along with FAQs on them, in this article!
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