Gas-Liquid Chromatography
We know that every Chromatography involves two types of phases named mobile phase and stationary phase. In Gas-Liquid Chromatography, the mobile is a gas like helium and the stationary phase is a liquid which is of high boiling point and is absorbed into a solid. The velocity of movement of a compound in the chromatography machine depends on the time it spent with the gas i.e. the mobile phase.
The working of Gas-Liquid Chromatography can be understood in the following steps:
Step 1: First the sample is injected using a syringe in a temperature-controlled oven. Here, the sample boils and moves into the column in gaseous form with the help of helium gas i.e. mobile phase.
Step 2: The column is packed with porous rock coated with a waxy polymer.
Step 3: The temperature of the column ranges between 50C to 250C. With the proceeding of analysis, the temperature starts rising.
Step 4: Inside the column, the sample may condense, it may dissolve in the liquid, or may remain in the gas. In general, the substance partitions itself between the gaseous mobile phase and the stationary liquid phase. Any molecule of the sample spends some of its time with the liquid stationary phase and some with the gaseous mobile phase.
Chromatography
Chromatography is the separation of mixtures by distribution between two or more immiscible phases. In chemical laboratories, where it is used for analysis, isolation, and purification, chromatography is the most used separation technique. It is also frequently used in the chemical process industry as a part of small and large-scale production.
There are many types of Chromatography, e.g. Adsorption Chromatography, Thin Layer Chromatography, Column Chromatography, Partition Chromatography, etc. Paper Chromatography is an example of Partition Chromatography. Let’s learn more about Chromatography, its meaning, principle, types, and applications along with FAQs on them, in this article!
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