Steps of Plant Tissue Culture
The steps involved in Plant Tissue Culture are:
- Step 1: Selection of Explants: Part of a Plant that holds regeneration potential and possesses the ability to give rise to the whole Plant is selected. These parts may include apical meristems, roots, embryo, leaf disc, leaf blade, anther, ovary, etc.
- Step 2: Sterilization and Inoculation of Explants: The explant is sterilized using a disinfecting solution and is introduced to the nutrient media composed of growth hormones and required nutrients. These components aid the healthy growth and multiplication of cells.
- Step 3: Root and Shoot formation: The explants give rise to callus, an undifferentiated mass of cells that is capable of division and differentiation. Plant hormone combinations are used to induce root and shoot development. This gives rise to plantlets.
- Step 4: Transfer of Plantlets: Plantlets are transferred to soil and are allowed to grow in the natural environment.
Plant Tissue Culture
Plant Tissue Culture became a new addition to the Plant breeding strategies that emerged around the 1950s. Tissue Culture emerged as a significant advancement in breeding methods when traditional breeding techniques failed to meet the demands of crops. It utilizes Plant components to produce several clones of the original Plant quickly. The method takes advantage of a Plant’s totipotency, which allows any cell from any section of the Plant to be used to create a brand-new Plant.
Table of Content
- What is Plant Tissue Culture?
- Steps of Plant Tissue Culture
- Types of Plant Tissue Culture
- What are the different Plant Tissue Culture mediums?
- Advantages of Plant Tissue Culture
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