Procedures of Test for Protein
The following test procedures are used:
Biuret Test for Protein
The steps of Biuret test are as follows.
- A clean and dry test tube should be taken.
- The sample is taken with the test tubes.
- 2ml of sodium hydroxide and 5 to 6 drops of copper sulfate solution are to be added.
- Then the test tube is shaken gently to mix the ingredients thoroughly and allow the mixture to stand for 4 – 5 minutes.
- If a bluish-violet color appears, it indicates the presence of protein.
Xanthoproteic Test
The steps of this test are given below.
- The sample is taken in a clean and dry test tube.
- A few drops of concentrated sulfuric acid are added and shaken the test tube.
- The test tube is heated gently on a Bunsen burner.
- If yellow precipitate forms, then it confirms the presence of protein
Millon’s Test
The steps of Millon’s test are as follows.
- 2-3 drops of Millon’s reagent are added to a test tube containing the sample and shaken well.
- At first a white precipitate forms which on heating turns into a brick red colour. This indicates the presence of protein.
Ninhydrin Test
The steps of the ninhydrin test are as follows.
- 1-2ml of ninhydrin solution is added to a test tube containing the sample and shaken it.
- Then the mixture is boiled.
- A deep blue or purple color appears, which confirms the presence of protein.
Protein and Test for Protein
Test for Protein deals with the details of protein including its structure, properties, classification, and function as well as the details of different tests to detect proteins in a sample. The identification test for protein explains the principle and theory of each test, along with the materials and apparatus required, the procedures, observation, inferences, and discussion of the results of the tests.
Protein is an important biomolecule that is made up of one or more amino acid chains. Plants and animals synthesize these biomolecules and obtain them by consuming the plants. In this article, we will look into the meaning, theory, procedure, observation, results, and precautions of tests for proteins.
Table of Content
- What are Proteins?
- Test for Protein to Confirm its Presence
- Aim of the Protein Test
- Theory of the Tests for Protein
- Apparatus Set up and Chemicals Required for Protein Test
- Procedures of Test for Protein
- Observation and Inference
- Results and Discussion of Test for Protein
- Precautions
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