Methods for the Creation of Transgenic Animals
Physical Transfection
This is the first method in which the interested gene is directly injected into the pronucleus of the fertilized ovum. This method was effective in mammals and was also applicable to a variety of species.
Chemical Transfection
The chemical method of gene transfection consists of transformation. In this method, when calcium phosphate was present the target DNA is taken up. Calcium phosphate co-precipitates or DNA takes DNA uptake.
Viral Vectors
Viral vectors are viruses that have the ability to infect the host cell. They transfect rDNA into the animal cells.
Bactofection
It is a method in which with the help of bacteria the interested gene is transferred into the target gene.
Retrovirus-mediated Gene Transfer
The gene is usually transferred by means of a vector and used as vectors to transfect the interested gene into the target genome.
Transgenic Animals Notes – Biotechnology And Its Application
CBSE Class 12- Biotechnology And Its Application – Transgenic Animals: When a foreign gene inserts into the genome of the animals to alter its DNA or the animals with a modified genome are Known as Transgenic Animals. It is a method that helps to improve the genetic traits of targeted animals. Transgenic animals are also called genetically modified organisms.
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