How are Restriction Enzymes Used in DNA Fingerprinting?
Short tandem repeats (STRs), a class of repeating components used in DNA fingerprinting, are examined in their pattern. STRs are non-coding DNA sequences that are located in the centromeric regions of chromosomes. STRs are therefore a subset of satellite DNA. Short nucleotide sequences (2–6 base pair) are therefore repeated sporadically in STRs. Since every person has a unique amount of STRs at a certain locus. Therefore, each person has a different DNA profile. In that regard, forensic investigations and paternity testing can both employ DNA fingerprinting to identify people.
What is DNA Fingerprinting?
DNA Fingerprinting is a technique used to identify individuals by analyzing their unique DNA patterns. Studying the DNA Fingerprinting steps and process helps in understanding genetic relationships, solving crimes, and identifying individuals based on their unique DNA profiles.
In this article, we will cover DNA fingerprinting applications, uses, and steps. You can also find DNA fingerprinting notes here.
Table of Content
- What is DNA Fingerprinting?
- DNA Fingerprinting Diagram
- DNA Fingerprinting Steps and Process
- Step 1: DNA Isolation
- Step 2: Amplification
- Step 3: DNA digestion by Restriction of Endonuclease Enzyme
- Step 4: DNA Fragment Separation
- Step 5: Separation of DNA Fragments
- Step 6: Transferring (blotting) the isolated DNA fragments from the gel to synthetic membranes such as nylon or nitrocellulose
- Step 7: Radiolabeled Probe Hybridizations
- Step 8: Hybridized DNA Fragment Detection
- Principle of DNA Fingerprinting
- DNA Fingerprinting Applications
- How are Restriction Enzymes Used in DNA Fingerprinting?
- How we can Create a DNA Profile Using Short Tandem Repeats (STR)
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