Process
- First, complete DNA will be isolated from a cell
- Then using restriction enzymes, we will divide the DNA into small fragments.
- Commonly used vectors known as BAC (bacterial artificial chromosomes) and YAC (yeast artificial chromosomes) will amplify the small fragments.
- Now, these fragments will get sequenced using an automated DNA sequencer that works on the principle of a method developed by Frederick Sanger.
- Now, these sequences will get rearranged based on some overlapping regions present in them.
- Now for sequencing, we required overlapping fragments.
- Now all the information related to the genome will store in a computer-based program.
- These successions were hence commented on and were allotted to every chromosome.
- Along these lines, the whole genome was sequenced and stored as a genome database on a computer.
- Genome mapping was the next goal that was accomplished with the assistance of microsatellites, i.e., the repetitive DNA succession.
Human Genome Project
Human Genome Project was the world’s largest collaborative biological project that gave us the ability to examine the full genetic manual for creating a human being in nature. HGP was international scientific research that mainly aims to determine the base pairs that make human DNA, as well as the identification, mapping, and sequencing of every gene in the human genome, both physically and functionally. The entire 3.3 billion base pair human genome was sequenced as part of a project that was successfully finished in 2003.
Contact Us