How to Calculate Isoelectric Point?
To calculate the isoelectric point we need to follow the below-listed steps:
- Assign pKa Values
- Calculating Their Average
Assign pKa values
The first step to calculate the isoelectric point is to assign the pKa values of the molecules. The pKa value is nothing but an ionizable group where a molecule is carrying a half-positive charge and a half-negative charge.
Calculating Their Average
After assigning the pKa values we need to calculate their average. This will provide us with the result of the isoelectric point(pI).
pI = (pKa +pKb)/2
Isoelectric Point
Isoelectric Point is the basic concept in the field of biochemistry and protein chemistry which plays an important role in understanding the behaviour of molecules in different environments. The isoelectric point is a property of an Amino Acid or protein that is defined as the pH at which a protein molecule carries an equal positive and negative charge.
In this article, we will learn about the isoelectric point as well as the formula for the isoelectric point and also provide a step-by-step guide to help you easily calculate the isoelectric point. So, let’s start with the definition of isoelectric point first.
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