Features of Cloning Vectors

The process of cloning is performed by using E. coli. Cloning of vectors consists of a system that permits function and maintenance in E.coli. Some of the other features are mentioned below:

• Vectors should be small in size so that they can easily integrate into the host cell.
• They should consists of a multiple sites for cloning and also consists of a restriction site for the insertion of target DNA.
• Integration of Donor DNA should not interfere in the replication procedure of cloning in vector.
• Cloning DNA consists of origin of replication so that they can self replicate inside the host cell.
• They should consists of selectable marker with antibiotic resistance gene which facilitates screening of recombinant organisms.

Cloning vectors are small piece of DNA molecule which is used to carry foreign DNA into the host cell which consists of a capability of self-replication and integration into the host cell. These vectors are used in the analysis of the molecular structure of DNA. The target DNA in the vector is integrated into the specific sites of this vector and ligated by DNA ligase. Phages and Plasmids are examples of cloning vectors.