DNA Cloning Vector
A DNA cloning vector is a small piece of DNA used as a vehicle to carry foreign genetic material into a host organism. Escherichia coli is a preffered choice. These vectors are designed to replicate independently within the host cell, allowing the inserted DNA to be copied along with the vector DNA. Plasmids are commonly used as DNA cloning vectors due to their ability to replicate autonomously.
Scientists manipulate these vectors in the laboratory by introducing desired DNA sequences into them using restriction enzymes and DNA ligase. Once the vector has been modified, it can be introduced into a host cell, where it will replicate, producing multiple copies of the inserted DNA sequence. This process enables researchers to produce large quantities of specific DNA fragments for various applications in biotechnology and genetic engineering.
DNA Cloning – An Overview
DNA cloning is an essential technique in molecular biology that enables researchers to produce multiple copies of a specific DNA sequence. It involves isolating a target DNA fragment and inserting it into a vector (such as a plasmid or a viral genome), which is then introduced into a host organism (usually bacteria or yeast) to produce multiple copies of the inserted DNA. In this article, find the DNA cloning notes and diagram.
Table of Content
- DNA Cloning Definition
- What is DNA Cloning?
- Diagram of DNA Cloning
- Methods of DNA Cloning
- Steps of DNA Cloning
- Application of Gene Cloning
- DNA Cloning Vector
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