Applications of Somatic Hybridization

Some of the applications of somatic hybridisation are:

  • Crop Improvement: Somatic hybridization is used to develop crop varieties with desirable traits such as disease resistance, stress tolerance, and improved yield.
  • Genetic Diversity Enhancement: By combining genetic material from different plant species, somatic hybridization contributes to the enrichment of genetic diversity in breeding programs.
  • Creation of Novel Varieties: This technique enables the creation of novel plant varieties with unique combinations of traits not found in traditional breeding programs.
  • Biotechnological Research: Somatic hybridization is instrumental in studying gene expression, functional genomics, and understanding the mechanisms of plant development and stress responses.
  • Disease Resistance: Disease-resistance genes have been able to spread from one plant to many others due to somatic hybridization. The spotted wilt virus, TMV, insect pests, and cold tolerance can no longer harm tomatoes.

What is Somatic Hybridization?

Somatic hybridization is a technique of fusing protoplasts from different plant species to create hybrid plants. It is different from conventional ways involving sexual hybridization because it does not need sexual reproduction. Instead, it combines traits from different plants without being limited by species barriers. In this article, we will cover somatic hybridization notes, steps, and its applications.

Table of Content

  • What is Somatic Hybridization?
  • What are the Stages of Somatic Hybridization?
  • Protoplast Fusion
  • Hybrid Cell Selection 
  • Identifying Hybrid Plants
  • Applications of Somatic Hybridization
  • Somatic Hybridization Examples
  • Advantages of Somatic Hybridization
  • Limitations of Somatic Hybridization

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What is Somatic Hybridization?

Somatic hybridization is a technique of creating a hybrid cell through the in vitro fusion of separate protoplasts, which can then grow into a hybrid plant. Sexual hybridization has long been the preferred strategy for enhancing the traits of domesticated plants. The major limitation of sexual hybridization is that it can only take place between closely related plant species. This limits the modifications that may be made to plants....

What are the Stages of Somatic Hybridization?

Somatic hybridization involves three processes. They are as follows:...

Protoplast Fusion

Since isolated protoplasts lack cell walls, in vitro fusion of these structures is simple. For protoplast fusion, there are no obstacles to incompatibility (at interspecific, inter-generic, or even at inter-kingdom levels). A protoplast fusion that includes combining protoplasts with two distinct genomes can be done naturally, mechanically, or artificially. These are explained below:...

Hybrid Cell Selection

A heterokaryon is produced by the fusion of just 20–25% of the protoplasts. The combination is made up of unfused protoplasts, heterokaryons, and homokaryons. From this varied mixture, methods are developed to choose the hybrid cells. Three choices are available for selection:...

Identifying Hybrid Plants

The initial somatic fusion of two distinct protoplasts must be confirmed as the source of any produced hybrids. The following list includes several methods of identification:...

Applications of Somatic Hybridization

Some of the applications of somatic hybridisation are:...

Somatic Hybridization Examples

Some of the examples of somatic hybridisation are:...

Advantages of Somatic Hybridization

The advantages are:...

Limitations of Somatic Hybridization

The limitations of somatic hybridisation are:...

Conclusion – Somatic Hybridization

Somatic hybridization offers a revolutionary approach to plant breeding. It enables the creation of hybrid plants with desirable traits by fusing protoplasts from different species. Unlike traditional methods, somatic hybridization does not rely on sexual reproduction, thus overcoming species barriers. While it presents numerous advantages such as enhanced genetic variation and the creation of novel varieties, somatic hybridization also faces limitations such as genetic instability and the occasional production of unhealthy plants. Despite these challenges, somatic hybridization remains a promising tool for meeting the growing demands of agriculture and biotechnology in the future....

FAQs on Somatic Hybridization

Which Chemical is Used in Somatic Hybridization?...

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